cellprofiler automated image analysis software Search Results


cellprofiler  (Broad Institute Inc)
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Broad Institute Inc cellprofiler
A majority of MCT14 positive neurons are non-GAD67. Double IHC on 7-µm paraffin sections with MCT14/NeuN and MCT14/GAD67 for determination of the type of neuron MCT14 is expressed in. MCT14 is seen in red , markers in green , and DAPI is seen in blue . a Colocalization of MCT14 with NeuN, a neuronal marker, in most MCT14-expressing cells. b Colocalization of MCT14 with neurons expressing GAD67, a marker for inhibitory neurons. c A total of six (n = 2) double IHC images with MCT14/NeunN and MCT14/GAD67 from retrosplenial cortex, hypothalamus and piriform cortex were collected and analyzed using a colocalization analysis pipeline from <t>CellProfiler.org</t> (Additional file : Figure S1). Fraction of MCT14-positive cells that also expressed respective marker was plotted. All images were acquired on a Zeiss AxioImager at ×20 magnification. Statistics were performed with Students’ t test, *p < 0.05
Cellprofiler, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A majority of MCT14 positive neurons are non-GAD67. Double IHC on 7-µm paraffin sections with MCT14/NeuN and MCT14/GAD67 for determination of the type of neuron MCT14 is expressed in. MCT14 is seen in red , markers in green , and DAPI is seen in blue . a Colocalization of MCT14 with NeuN, a neuronal marker, in most MCT14-expressing cells. b Colocalization of MCT14 with neurons expressing GAD67, a marker for inhibitory neurons. c A total of six (n = 2) double IHC images with MCT14/NeunN and MCT14/GAD67 from retrosplenial cortex, hypothalamus and piriform cortex were collected and analyzed using a colocalization analysis pipeline from CellProfiler.org (Additional file : Figure S1). Fraction of MCT14-positive cells that also expressed respective marker was plotted. All images were acquired on a Zeiss AxioImager at ×20 magnification. Statistics were performed with Students’ t test, *p < 0.05

Journal: BMC Neuroscience

Article Title: Histological characterization of orphan transporter MCT14 (SLC16A14) shows abundant expression in mouse CNS and kidney

doi: 10.1186/s12868-016-0274-7

Figure Lengend Snippet: A majority of MCT14 positive neurons are non-GAD67. Double IHC on 7-µm paraffin sections with MCT14/NeuN and MCT14/GAD67 for determination of the type of neuron MCT14 is expressed in. MCT14 is seen in red , markers in green , and DAPI is seen in blue . a Colocalization of MCT14 with NeuN, a neuronal marker, in most MCT14-expressing cells. b Colocalization of MCT14 with neurons expressing GAD67, a marker for inhibitory neurons. c A total of six (n = 2) double IHC images with MCT14/NeunN and MCT14/GAD67 from retrosplenial cortex, hypothalamus and piriform cortex were collected and analyzed using a colocalization analysis pipeline from CellProfiler.org (Additional file : Figure S1). Fraction of MCT14-positive cells that also expressed respective marker was plotted. All images were acquired on a Zeiss AxioImager at ×20 magnification. Statistics were performed with Students’ t test, *p < 0.05

Article Snippet: All images were analyzed using a specialized pipeline in the automated open-source cell segmentation software CellProfiler (Additional file : Figure S1) (Broad Institute Imaging Platform, Cambridge, MA, USA) [ ].

Techniques: Marker, Expressing